The protocol describes a method for capture of secreted hexahistidine-tagged proteins using expanded-bed adsorption immobilized-metal affinity chromatography. The starting material for the procedure ...
An automated FAC-FD system (FAC-1) is illustrated in Figure 2a. It consists of two isocratic pumps, an auto-sampling system, a column oven, two miniature columns connected to an FL detector and a ...
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