Real-time PCR or quantitative PCR (Polymerase Chain Reaction) is an advancement of the standard PCR technique, which enables continuous monitoring of the reaction and collection of data in real time.
With the launch of its new ProbeLibrary kits, Danish biotech firm Exiqon http://www.exiqon.com seeks to simplify real-time PCR. Typically 30 to 35 bases long, PCR ...
image: Eprobe®, a highly efficient and reliable fluorescent probe for PCR DNA amplification techniques and DNA analysis in hybridization experiments, has been developed by researchers from RIKEN and ...
How it Works: Real Time PCR Brendan Maher The instrumentation is basic: a thermal cycler for amplification, a light source for excitation of fluorescent probes (see chemistries below), a camera for ...
What is real-time quantitative PCR? qPCR uses one of two methods to detect the changes to DNA amplification: use of fluorescent dyes or sequence-specific DNA probes. qPCR is normally performed in a ...
With the introduction of fluorogenic probes and fluorometric thermal cyclers that enable the monitoring of amplification reactions in real time, the sensitivity and precision of gene expression ...
Eurogentec obtained a license from Applied Biosystems to manufacture and sell dual-labeled oligonucleotide probes worldwide for use in the 5´ Nuclease process. This process is one of the key ...
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