Immobilized metal affinity chromatography (IMAC) separation is commonly used to purify proteins of interest from a crude mixture. A variety of companies offer nickel-based beads of uneven shapes to ...
Immobilized metal affinity chromatography (IMAC) is a reliable method for isolation of recombinant protein following over-expression in Escherichia coli. The polyhistidine tag (6–10 consecutive amino ...
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