The enzyme-linked immunosorbent spot (ELISpot) is a type of assay that focuses on quantitatively measuring the frequency of cytokine secretion for a single cell.

Jul 3, 2025 · ELISPOT is a highly sensitive assay designed to detect secreted proteins from individual cells, making it ideal for immunological studies. Developed as an extension of ELISA, ELISPOT …

EliSpot (Enzyme linked immuno spot) is a technology, to evaluate a functional immnue answer of T or B cells to different antigens. On this site we try to answer all open questions you may have regarding …

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

May 23, 2022 · ELISpot has evolved from a research tool to an important clinical assay. Recent studies of cancer vaccines tested in a variety of malignancies have suggested that ELIspot may be a useful …

ELISPOT is the most sensitive technique available for the detection of immune cells which secrete specific signature proteins such as antibodies or cytokines. It is the state-of-the-art method for the …

Nov 10, 2022 · ELISpot is an immunoassay used to quantify analyte-secreting cells. Cytokines, immunoglobulins, or other target proteins secreted by cells are captured by specific antibodies …

Key issues. Enzyme-linked immunosorbent spot (ELISpot) is a highly flexible assay and can be adapted to multiple readout formats. ELISpot assays are quantitative and measure key cellular functions of …

The cytokine ELISpot assay has been widely applied to investigate specific immune responses in infections, cancer, allergies and autoimmune diseases. It has also become a standard tool in the …

The ELISpot assay is a hybrid assay that combines the features of a mixed lymphocyte reaction (MLR) assay and an enzyme-linked immunosorbent assay (ELISA). In it's main principle, the ELISpot …