Project Coral 2025 part 2 - The freezing! After collecting the coral and sea urchin semen, it is time to freeze! Firstly, we added cryoprotectant solutions to each sample to protect the sperm cells during the freezing process. We then used our portable freezing devices (designed by Mary Hagedorn's research group at the Smithsonian Conservation Biology Institute and 3D printed by Rob Lickley) to freeze the samples at a controlled rate until they reached -80⁰C. After this, they were submerged into
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This sped-up video illustrates the freezing and thawing process for hibernating wood frogs. When winter brings fall to a close, these frogs hunker down in dead leaves and moss that cover the ground, which serve as their shelter for the months ahead. As the cold sets in, frost creeps across the plant matter and reaches the immobile frogs, who then begin the process of releasing a cryoprotectant that keeps their bodies from drying out as they freeze. Making sure ice doesn’t form in their cells is
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